Utilization of sugarcane bagasse for enhancement production of fibrinolytic enzyme using statistical approach

The purpose of this investigation was to produce fibrinolytic enzyme by utilizing sugarcane bagasse as low cost
substrate through solid state fermentation in an effective way. The potential producer of microorganism, Alcaligenes
aquatilis PJS_1 was isolated and identified from slaughter house soil samples. In small scale level, higher
production of microbial fibrinolytic enzyme was observed when the conditions provided like Glucose for carbon,
Casein for nitrogen and (NH4)2SO4 for mineral sources when maintained at 60 h with 100% moisture. The
sugarcane bagasse medium with glucose and casein was found to have optimum production when incubated for
60 h at 35◦C. The culture conditions were optimized to improve the production of fibrinolytic enzyme (3832 U
ml� 1) from Alcaligenes aquatilis PJS_1 through statistical approach. Blood clotting assay was performed to
determine its anticoagulant property. The fibrinolytic property of the synthesised enzyme was confirmed when it
dissolved blood clot sample effectively. Thus, the enzyme it could be used in medicine to produce as a thrombolytic
agent.