Exploration of new effective fibrinolytic agent from Alcaligenes aquatilis PJS_1 from polluted soil

Aim:
Methodology:
Results:
Interpretation:
To isolate and identify Alcaligenes aquatilis PJS_1 from slaughter house soil samples for production of enzymatic fibrinolytic agent production.
Fibrinolytic enzyme producing bacterium was isolated from slaughter house soil samples and identified by biochemical tests and 16S
rRNA sequencing. The
f i b r i n o l y t i c e n z y m e
production media was
optimized by various
factors like energy sources,
pH and temperature.
Bioreactor used in the
experiment was designed
with suitable parameters for
effective production and
purification is by gel
filtration chromatography.
Blood clotting assay was
performed to determine its
anticoagulant property.
The isolated
e n z y m e p r o d u c i n g
bacterium was identified as
Alcaligenes aquatilis
PJS_1. The medium with
fructose and urea at pH 7.0
was found to have optimum production when incubated for 24 hr at 37ºC. The crude enzyme was purified by acetone precipitation followed by gel
filtration chromatography. The enzyme showed a final specific activity of 629.32 Umg-1 with of 88.24% yield.
The present study provides information that the enzyme produced by Alcaligenes aquatilis PJS_1 acts as an effective fibrinolytic agent.
Key words: Alcaligenes sp., Anticoagulant, Bioreactor, Fibrinolytic enzyme