Sequestration and purification of essential enzyme from Pleurotus florida compost waste and its application studies

Spent oyster mushroom (Pleurotus florida) compost as tremendous source for isolation of industrial significant
enzymes such as amylase, protease, and cellulase. This study was conducted to achieve efficient extraction of
lignocellulolytic enzymes amylase (EC 3.2.1.1), cellulase (EC 3.2.1.4), and protease (EC 3.4.21.14) from spent oyster
mushroom (P. florida) compost waste. Optimal enzyme recovery was achieved when spent oyster mushroom compost
wastes and concentrated by acetone precipitation. The purification was performed by column chromatography. The
enzymes such as cellulase, amylase, and protease released from oyster mushroom (P. florida) compost waste were
showed activities of 15.78 U/ml, 3.42 U/ml, and 0.042 U/ml, respectively. These were utilized in various industrial
and environmental applications such as starch processing in potato waste from food industry using amylase and
biotreatment of cotton waste using cellulase.