Optimization of production of Extracellular proteolytic keratinase using
Streptomyces sp. isolated from poultry plant soil

Mohanasundaram, S; Doss, VA; Prabhaharan, CTA; Joseph, J; Venugopal, K; Agilandeshwari, P; Sudansuhaa, C; Balaganesh, R; Ramkumar, R; Rajaruban, MDS; Balaji, B

Optimization of production of Extracellular proteolytic keratinase using Streptomyces sp. isolated from poultry plant soil

Mohanasundaram, S and Doss, VA and Prabhaharan, CTA and Joseph, J and Venugopal, K and Agilandeshwari, P and Sudansuhaa, C and Balaganesh, R and Ramkumar, R and Rajaruban, MDS and Balaji, B Optimization of production of Extracellular proteolytic keratinase using Streptomyces sp. isolated from poultry plant soil. International Journal of Research in Pharmaceutical Sciences.

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Abstract

In this study, Proteolytic keratinase was observed from Streptomyces sp., isolated from poultry plant soil and purified
by ammonium sulfate fractionation method followed by Gel filtration chromatography. The purified enzyme
was further characterized partially which covers the influence of pH, temperature, incubation time, nitrogen
sources and metal ions on the activity of proteolytic keratinase. From the results, it was observed that, proteolytic
keratinase isolated from the Streptomyces sp. enumerated from the poultry plant soil showed maximal activity at
pH 8.0 and at temperature 50oC within 55mins. It was also found that, at the minimum concentration of 2mM
BaCl.2H2O, CuSO4.5H2O and MgCl2, it showed highest keratinase activity.

Item Type:	Article
Subjects:	Biotechnology > Bioprocess Engineering
Divisions:	Biotechnology
Depositing User:	Unnamed user with email techsupport@mosys.org
Date Deposited:	30 Oct 2026 06:16
Last Modified:	30 Oct 2026 06:16
URI:	https://ir.vcenggw.ac.in/id/eprint/549

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